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GSE53960: rat RNA-Seq transcriptomic Bodymap

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Results for Mafa

Z-value: 0.58

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Transcription factors associated with Mafa

Gene Symbol Gene ID Gene Info
ENSRNOG00000007668 MAF bZIP transcription factor A

Activity-expression correlation:

GenePromoterPearson corr. coef.P-valuePlot
Mafarn6_v1_chr7_-_116781766_1167817660.172.3e-03Click!

Activity profile of Mafa motif

Sorted Z-values of Mafa motif

Promoter Log-likelihood Transcript Gene Gene Info
chr10_+_53818818 29.24 ENSRNOT00000057260
myosin heavy chain 8
chr8_+_32018560 16.52 ENSRNOT00000007358
ADAM metallopeptidase with thrombospondin type 1 motif, 8
chr1_-_174495258 16.09 ENSRNOT00000052377
ENSRNOT00000086349
signal peptide, CUB domain and EGF like domain containing 2
chr16_-_18766174 16.06 ENSRNOT00000084813
surfactant protein D
chr10_+_73333119 14.90 ENSRNOT00000004736
T-box 4
chr11_+_17538063 14.43 ENSRNOT00000031889
ENSRNOT00000090878
chondrolectin
chr3_-_117766120 13.26 ENSRNOT00000056022
fibrillin 1
chr5_+_173288447 11.66 ENSRNOT00000091205
ENSRNOT00000067252
matrix remodeling associated 8
chr10_+_94170766 10.79 ENSRNOT00000010627
angiotensin I converting enzyme
chr1_+_272799784 10.10 ENSRNOT00000016052
insulin 1
chr16_-_69132584 10.02 ENSRNOT00000017776
adhesion G protein-coupled receptor A2
chr3_+_15560712 9.98 ENSRNOT00000010218
prostaglandin-endoperoxide synthase 1
chr20_-_21689553 9.36 ENSRNOT00000038095
transmembrane protein 26
chr19_-_11341863 8.45 ENSRNOT00000025694
metallothionein 4
chr3_-_7203420 7.60 ENSRNOT00000015236
growth factor independent 1B transcriptional repressor
chr1_-_254735548 7.52 ENSRNOT00000025258
ankyrin repeat domain 1
chr3_+_6430201 7.39 ENSRNOT00000086352
collagen type V alpha 1 chain
chr1_+_282265370 7.30 ENSRNOT00000015687
G protein-coupled receptor kinase 5
chr10_+_36098051 6.89 ENSRNOT00000083971
ADAM metallopeptidase with thrombospondin type 1 motif, 2
chr1_-_215858034 6.71 ENSRNOT00000027656
insulin 2
chr20_-_12938891 6.26 ENSRNOT00000017141
similar to Protein C21orf58
chr11_+_57265732 5.82 ENSRNOT00000093174
pleckstrin homology-like domain, family B, member 2
chr20_-_32139789 4.94 ENSRNOT00000078140
serglycin
chr18_+_30172740 4.90 ENSRNOT00000027340
protocadherin alpha 4
chr7_-_12424367 4.12 ENSRNOT00000060698
midnolin
chr20_+_4329811 4.09 ENSRNOT00000000513
notch 4
chr15_+_105851542 4.04 ENSRNOT00000086959
RAS related protein 2a
chr1_+_221099998 3.71 ENSRNOT00000028262
latent transforming growth factor beta binding protein 3
chr7_-_117267402 3.71 ENSRNOT00000088945
plectin
chr12_-_22726982 3.68 ENSRNOT00000001921
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
chr7_-_117267803 3.49 ENSRNOT00000082271
plectin
chr6_+_102392828 3.31 ENSRNOT00000089162
retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
chr1_-_145931583 3.25 ENSRNOT00000016433
cilia and flagella associated protein 161
chr9_+_16003058 2.94 ENSRNOT00000081621
ENSRNOT00000021158
ubiquitin protein ligase E3 component n-recognin 2
chr13_+_52147555 2.72 ENSRNOT00000084766
leiomodin 1
chr20_-_7930929 2.71 ENSRNOT00000000607
TEA domain transcription factor 3
chr20_+_5509059 2.42 ENSRNOT00000065349
kinesin family member C1
chr9_+_16543688 2.39 ENSRNOT00000021868
canopy FGF signaling regulator 3
chr6_-_138909105 2.39 ENSRNOT00000087855

chr1_-_224974203 2.32 ENSRNOT00000065694
transmembrane protein 179B
chrX_+_22247088 2.29 ENSRNOT00000079802
IQ motif and Sec7 domain 2
chr8_-_116438038 2.25 ENSRNOT00000023791
G protein subunit alpha transducin 1
chr18_+_65814026 2.24 ENSRNOT00000016112
methyl-CpG binding domain protein 2
chr1_-_225128740 2.15 ENSRNOT00000026897
retinal outer segment membrane protein 1
chr1_-_167210919 2.11 ENSRNOT00000027507
cholinergic receptor nicotinic alpha 10 subunit
chr2_-_27949066 2.04 ENSRNOT00000081472
ENSRNOT00000022138
NSA2 ribosome biogenesis homolog
chrX_-_10413984 2.03 ENSRNOT00000039551
ENSRNOT00000091448
DEAD-box helicase 3, X-linked
chr5_+_26493212 2.01 ENSRNOT00000061328
triple QxxK/R motif containing
chr10_+_83081168 1.94 ENSRNOT00000035023
tachykinin 4 (hemokinin)
chr1_+_245237736 1.90 ENSRNOT00000035814
very low density lipoprotein receptor
chr1_+_162083030 1.84 ENSRNOT00000016361
GRB2-associated binding protein 2
chr20_-_5056474 1.81 ENSRNOT00000076375
ENSRNOT00000037191
ENSRNOT00000076863
immunoreceptor tyrosine-based inhibitory motif (ITIM) containing platelet receptor
chr1_-_170594168 1.80 ENSRNOT00000026280
tripeptidyl peptidase 1
chrX_-_111102464 1.80 ENSRNOT00000084176
ripply transcriptional repressor 1
chr2_+_185524774 1.78 ENSRNOT00000089338
LPS responsive beige-like anchor protein
chr10_+_14828597 1.57 ENSRNOT00000025434
tektin 4
chr13_-_74276964 1.33 ENSRNOT00000005726
torsin family 3, member A
chr7_+_13318533 1.17 ENSRNOT00000034545
similar to zinc finger protein 84 (HPF2)
chr8_-_107602263 1.16 ENSRNOT00000017658
extended synaptotagmin 3
chr6_-_127653124 1.05 ENSRNOT00000047324
serpin family A member 11
chr13_-_37287458 0.95 ENSRNOT00000003391
insulin induced gene 2
chr18_+_44810388 0.94 ENSRNOT00000021646
ENSRNOT00000089750
hydroxysteroid (17-beta) dehydrogenase 4
chrX_+_123913072 0.88 ENSRNOT00000071376
reproductive homeobox 5
chr1_-_213987053 0.86 ENSRNOT00000072774
p53-induced protein with a death domain-like
chr5_+_3955227 0.70 ENSRNOT00000009908
musculin
chr4_-_144318580 0.69 ENSRNOT00000007591
ssu-2 homolog
chr1_-_87221826 0.66 ENSRNOT00000046611
ENSRNOT00000028006
serine peptidase inhibitor, Kunitz type, 2
chr20_+_10438444 0.50 ENSRNOT00000071248
ENSRNOT00000075545
crystallin, alpha A
chr3_-_60813869 0.46 ENSRNOT00000058234
ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9)
chr8_+_75625174 0.42 ENSRNOT00000013742
interactor of little elongation complex ELL subunit 2
chr5_-_58113553 0.38 ENSRNOT00000046589
AT-rich interaction domain 3C
chr10_+_89174684 0.31 ENSRNOT00000043754
vacuolar protein sorting 25
chr4_+_96252798 0.11 ENSRNOT00000008947
similar to RIKEN cDNA C130060K24 gene
chr8_+_42284654 0.10 ENSRNOT00000043810
olfactory receptor 8B3-like
chr3_+_176865156 0.09 ENSRNOT00000019084
zinc finger CCCH-type and G-patch domain containing
chr14_+_114126943 0.01 ENSRNOT00000041638
ENSRNOT00000006443
ENSRNOT00000006957
reticulon 4

Network of associatons between targets according to the STRING database.

First level regulatory network of Mafa

PNG image of the network

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Gene Ontology Analysis

Gene overrepresentation in biological process category:

Log-likelihood per target  Total log-likelihood Term Description
4.4 13.3 GO:2001205 negative regulation of osteoclast development(GO:2001205)
3.3 10.0 GO:0090210 regulation of establishment of blood-brain barrier(GO:0090210)
3.2 16.1 GO:1902732 positive regulation of chondrocyte proliferation(GO:1902732)
3.2 16.1 GO:0050828 regulation of liquid surface tension(GO:0050828)
2.9 11.7 GO:0060857 establishment of glial blood-brain barrier(GO:0060857)
2.7 10.8 GO:1903596 regulation of gap junction assembly(GO:1903596)
2.7 29.2 GO:0030049 muscle filament sliding(GO:0030049)
2.2 6.7 GO:0033861 negative regulation of NAD(P)H oxidase activity(GO:0033861)
2.0 10.0 GO:0019371 cyclooxygenase pathway(GO:0019371)
1.8 7.4 GO:1903225 negative regulation of endodermal cell differentiation(GO:1903225)
1.6 4.9 GO:0033364 mast cell secretory granule organization(GO:0033364)
1.2 3.7 GO:1902460 regulation of mesenchymal stem cell proliferation(GO:1902460) positive regulation of mesenchymal stem cell proliferation(GO:1902462)
1.2 3.7 GO:0046946 hydroxylysine metabolic process(GO:0046946) hydroxylysine biosynthetic process(GO:0046947)
1.2 9.2 GO:0007217 tachykinin receptor signaling pathway(GO:0007217)
1.0 4.1 GO:1903849 regulation of aorta morphogenesis(GO:1903847) positive regulation of aorta morphogenesis(GO:1903849)
1.0 7.6 GO:0051572 negative regulation of histone H3-K4 methylation(GO:0051572)
0.8 4.1 GO:0033132 negative regulation of glucokinase activity(GO:0033132) negative regulation of hexokinase activity(GO:1903300)
0.6 5.8 GO:1903690 negative regulation of wound healing, spreading of epidermal cells(GO:1903690)
0.5 1.9 GO:0034436 glycoprotein transport(GO:0034436)
0.4 7.5 GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator(GO:0043517)
0.4 7.2 GO:0031581 hemidesmosome assembly(GO:0031581)
0.4 2.3 GO:0051343 sensory perception of umami taste(GO:0050917) positive regulation of cyclic-nucleotide phosphodiesterase activity(GO:0051343)
0.4 2.9 GO:0071233 cellular response to leucine(GO:0071233)
0.3 2.7 GO:0051694 pointed-end actin filament capping(GO:0051694)
0.3 1.6 GO:0080154 regulation of fertilization(GO:0080154)
0.3 0.9 GO:0036112 medium-chain fatty-acyl-CoA metabolic process(GO:0036112)
0.3 2.0 GO:0042256 mature ribosome assembly(GO:0042256) protein localization to cytoplasmic stress granule(GO:1903608)
0.3 4.0 GO:0045198 establishment of epithelial cell apical/basal polarity(GO:0045198)
0.3 2.7 GO:0055059 asymmetric neuroblast division(GO:0055059)
0.3 2.3 GO:1900454 positive regulation of long term synaptic depression(GO:1900454)
0.2 0.7 GO:0014707 branchiomeric skeletal muscle development(GO:0014707)
0.2 2.2 GO:0035563 positive regulation of chromatin binding(GO:0035563)
0.2 0.7 GO:0060671 epithelial cell differentiation involved in embryonic placenta development(GO:0060671) epithelial cell morphogenesis involved in placental branching(GO:0060672)
0.1 6.9 GO:0030199 collagen fibril organization(GO:0030199)
0.1 1.8 GO:0032525 somite rostral/caudal axis specification(GO:0032525)
0.1 2.2 GO:0061298 retina vasculature development in camera-type eye(GO:0061298)
0.1 1.8 GO:0043306 positive regulation of mast cell activation involved in immune response(GO:0033008) positive regulation of mast cell degranulation(GO:0043306)
0.1 14.9 GO:0035113 embryonic limb morphogenesis(GO:0030326) embryonic appendage morphogenesis(GO:0035113)
0.1 1.0 GO:0060363 cranial suture morphogenesis(GO:0060363)
0.1 2.1 GO:0050910 detection of mechanical stimulus involved in sensory perception of sound(GO:0050910)
0.1 3.3 GO:0042572 retinol metabolic process(GO:0042572)
0.1 0.5 GO:0070309 lens fiber cell morphogenesis(GO:0070309)
0.1 0.3 GO:0043328 protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway(GO:0043328)
0.1 2.0 GO:0000470 maturation of LSU-rRNA(GO:0000470)
0.1 0.4 GO:0042796 snRNA transcription from RNA polymerase III promoter(GO:0042796)
0.1 2.4 GO:0007080 mitotic metaphase plate congression(GO:0007080)
0.1 1.8 GO:0043171 peptide catabolic process(GO:0043171)
0.1 9.8 GO:0051591 response to cAMP(GO:0051591)
0.0 4.9 GO:0042476 odontogenesis(GO:0042476)
0.0 0.5 GO:0015986 energy coupled proton transport, down electrochemical gradient(GO:0015985) ATP synthesis coupled proton transport(GO:0015986)

Gene overrepresentation in cellular component category:

Log-likelihood per target  Total log-likelihood Term Description
1.9 29.2 GO:0032982 myosin filament(GO:0032982)
1.8 7.4 GO:0005588 collagen type V trimer(GO:0005588)
1.2 13.3 GO:0043205 microfibril(GO:0001527) fibril(GO:0043205)
0.5 12.4 GO:0097225 sperm midpiece(GO:0097225)
0.4 16.1 GO:0005771 multivesicular body(GO:0005771)
0.4 5.8 GO:0045180 basal cortex(GO:0045180)
0.3 7.2 GO:0030056 hemidesmosome(GO:0030056)
0.3 1.0 GO:0032937 SREBP-SCAP-Insig complex(GO:0032937)
0.3 6.7 GO:0005732 small nucleolar ribonucleoprotein complex(GO:0005732)
0.2 6.1 GO:0042629 mast cell granule(GO:0042629)
0.1 2.2 GO:0042622 photoreceptor outer segment membrane(GO:0042622)
0.1 3.7 GO:0030867 rough endoplasmic reticulum membrane(GO:0030867)
0.1 8.8 GO:0001750 photoreceptor outer segment(GO:0001750)
0.1 2.0 GO:0005852 eukaryotic translation initiation factor 3 complex(GO:0005852)
0.1 2.1 GO:0005892 acetylcholine-gated channel complex(GO:0005892)
0.1 0.4 GO:0035363 histone locus body(GO:0035363)
0.1 0.3 GO:0000814 ESCRT II complex(GO:0000814)
0.1 1.9 GO:0034385 very-low-density lipoprotein particle(GO:0034361) triglyceride-rich lipoprotein particle(GO:0034385)
0.1 1.2 GO:0044232 organelle membrane contact site(GO:0044232)
0.1 4.0 GO:0055038 recycling endosome membrane(GO:0055038)
0.1 2.0 GO:0030687 preribosome, large subunit precursor(GO:0030687)
0.1 7.6 GO:0016363 nuclear matrix(GO:0016363)
0.1 7.5 GO:0001650 fibrillar center(GO:0001650)
0.0 17.0 GO:0005578 proteinaceous extracellular matrix(GO:0005578)
0.0 31.5 GO:0009986 cell surface(GO:0009986)
0.0 2.4 GO:0005871 kinesin complex(GO:0005871)
0.0 0.5 GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)(GO:0000276)
0.0 10.1 GO:0030141 secretory granule(GO:0030141)
0.0 2.2 GO:0000118 histone deacetylase complex(GO:0000118)
0.0 1.8 GO:0042470 melanosome(GO:0042470) pigment granule(GO:0048770)
0.0 2.7 GO:0005884 actin filament(GO:0005884)
0.0 1.3 GO:0005788 endoplasmic reticulum lumen(GO:0005788)
0.0 2.8 GO:0031012 extracellular matrix(GO:0031012)
0.0 10.9 GO:0048471 perinuclear region of cytoplasm(GO:0048471)
0.0 4.9 GO:0016607 nuclear speck(GO:0016607)

Gene overrepresentation in molecular function category:

Log-likelihood per target  Total log-likelihood Term Description
4.0 16.1 GO:0097108 hedgehog family protein binding(GO:0097108)
3.6 10.8 GO:0070573 bradykinin receptor binding(GO:0031711) metallodipeptidase activity(GO:0070573)
1.3 29.2 GO:0000146 microfilament motor activity(GO:0000146)
1.2 3.7 GO:0008475 procollagen-lysine 5-dioxygenase activity(GO:0008475) procollagen glucosyltransferase activity(GO:0033823)
1.2 7.3 GO:0047696 beta-adrenergic receptor kinase activity(GO:0047696)
0.6 16.1 GO:0001530 lipopolysaccharide binding(GO:0001530)
0.6 1.9 GO:0034437 glycoprotein transporter activity(GO:0034437)
0.6 1.8 GO:0008240 tripeptidyl-peptidase activity(GO:0008240)
0.5 7.5 GO:0031432 titin binding(GO:0031432)
0.5 7.4 GO:0048407 platelet-derived growth factor binding(GO:0048407)
0.5 14.4 GO:0005540 hyaluronic acid binding(GO:0005540)
0.5 2.9 GO:0070728 leucine binding(GO:0070728)
0.4 16.8 GO:0005158 insulin receptor binding(GO:0005158)
0.4 1.8 GO:0005068 transmembrane receptor protein tyrosine kinase adaptor activity(GO:0005068)
0.3 2.2 GO:0035197 siRNA binding(GO:0035197)
0.3 0.9 GO:0016508 long-chain-enoyl-CoA hydratase activity(GO:0016508)
0.3 2.0 GO:0033592 RNA strand annealing activity(GO:0033592)
0.3 13.3 GO:0005201 extracellular matrix structural constituent(GO:0005201)
0.2 10.0 GO:0004601 peroxidase activity(GO:0004601)
0.2 7.2 GO:0030506 ankyrin binding(GO:0030506)
0.2 23.4 GO:0004222 metalloendopeptidase activity(GO:0004222)
0.2 4.1 GO:0005112 Notch binding(GO:0005112)
0.2 2.7 GO:0005523 tropomyosin binding(GO:0005523)
0.2 3.3 GO:0004745 retinol dehydrogenase activity(GO:0004745)
0.1 3.7 GO:0050431 transforming growth factor beta binding(GO:0050431)
0.1 2.1 GO:0022848 acetylcholine-gated cation channel activity(GO:0022848)
0.1 2.3 GO:0005086 ARF guanyl-nucleotide exchange factor activity(GO:0005086)
0.1 2.3 GO:0031683 G-protein beta/gamma-subunit complex binding(GO:0031683)
0.1 4.9 GO:0005518 collagen binding(GO:0005518)
0.1 10.3 GO:0001085 RNA polymerase II transcription factor binding(GO:0001085)
0.1 4.0 GO:0019003 GDP binding(GO:0019003)
0.1 0.7 GO:0001206 transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding(GO:0001206)
0.0 2.4 GO:0003777 microtubule motor activity(GO:0003777)
0.0 1.3 GO:0016836 hydro-lyase activity(GO:0016836)
0.0 0.5 GO:0046933 proton-transporting ATP synthase activity, rotational mechanism(GO:0046933)
0.0 0.5 GO:0005212 structural constituent of eye lens(GO:0005212)
0.0 5.8 GO:0045296 cadherin binding(GO:0045296)
0.0 14.5 GO:0001071 nucleic acid binding transcription factor activity(GO:0001071) transcription factor activity, sequence-specific DNA binding(GO:0003700)

Gene overrepresentation in curated gene sets: canonical pathways category:

Log-likelihood per target  Total log-likelihood Term Description
0.6 13.3 PID INTEGRIN5 PATHWAY Beta5 beta6 beta7 and beta8 integrin cell surface interactions
0.6 10.8 SA MMP CYTOKINE CONNECTION Cytokines can induce activation of matrix metalloproteinases, which degrade extracellular matrix.
0.3 16.1 PID HNF3A PATHWAY FOXA1 transcription factor network
0.2 5.6 PID RHODOPSIN PATHWAY Visual signal transduction: Rods
0.2 7.3 PID ALPHA SYNUCLEIN PATHWAY Alpha-synuclein signaling
0.2 7.4 PID SYNDECAN 1 PATHWAY Syndecan-1-mediated signaling events
0.1 26.0 NABA ECM REGULATORS Genes encoding enzymes and their regulators involved in the remodeling of the extracellular matrix
0.1 3.6 NABA PROTEOGLYCANS Genes encoding proteoglycans
0.1 1.8 PID IL3 PATHWAY IL3-mediated signaling events
0.1 4.1 PID NOTCH PATHWAY Notch signaling pathway
0.0 1.6 PID REELIN PATHWAY Reelin signaling pathway
0.0 2.2 PID HDAC CLASSI PATHWAY Signaling events mediated by HDAC Class I
0.0 8.3 NABA SECRETED FACTORS Genes encoding secreted soluble factors
0.0 3.7 NABA ECM GLYCOPROTEINS Genes encoding structural ECM glycoproteins

Gene overrepresentation in curated gene sets: REACTOME pathways category:

Log-likelihood per target  Total log-likelihood Term Description
0.7 29.2 REACTOME STRIATED MUSCLE CONTRACTION Genes involved in Striated Muscle Contraction
0.3 4.1 REACTOME SIGNALING BY NOTCH4 Genes involved in Signaling by NOTCH4
0.3 18.0 REACTOME COLLAGEN FORMATION Genes involved in Collagen formation
0.3 7.2 REACTOME CASPASE MEDIATED CLEAVAGE OF CYTOSKELETAL PROTEINS Genes involved in Caspase-mediated cleavage of cytoskeletal proteins
0.2 10.0 REACTOME PHASE1 FUNCTIONALIZATION OF COMPOUNDS Genes involved in Phase 1 - Functionalization of compounds
0.2 2.4 REACTOME TRAFFICKING AND PROCESSING OF ENDOSOMAL TLR Genes involved in Trafficking and processing of endosomal TLR
0.2 13.3 REACTOME INTEGRIN CELL SURFACE INTERACTIONS Genes involved in Integrin cell surface interactions
0.2 2.7 REACTOME SMOOTH MUSCLE CONTRACTION Genes involved in Smooth Muscle Contraction
0.1 2.7 REACTOME YAP1 AND WWTR1 TAZ STIMULATED GENE EXPRESSION Genes involved in YAP1- and WWTR1 (TAZ)-stimulated gene expression
0.1 0.9 REACTOME ALPHA LINOLENIC ACID ALA METABOLISM Genes involved in alpha-linolenic acid (ALA) metabolism
0.1 2.2 REACTOME RNA POL I PROMOTER OPENING Genes involved in RNA Polymerase I Promoter Opening
0.1 7.5 REACTOME PPARA ACTIVATES GENE EXPRESSION Genes involved in PPARA Activates Gene Expression
0.1 2.4 REACTOME KINESINS Genes involved in Kinesins
0.1 2.3 REACTOME G PROTEIN ACTIVATION Genes involved in G-protein activation
0.1 1.8 REACTOME SIGNALING BY FGFR1 FUSION MUTANTS Genes involved in Signaling by FGFR1 fusion mutants
0.1 4.9 REACTOME RESPONSE TO ELEVATED PLATELET CYTOSOLIC CA2 Genes involved in Response to elevated platelet cytosolic Ca2+
0.0 7.3 REACTOME G ALPHA Q SIGNALLING EVENTS Genes involved in G alpha (q) signalling events
0.0 1.1 REACTOME ACTIVATION OF CHAPERONE GENES BY XBP1S Genes involved in Activation of Chaperone Genes by XBP1(S)
0.0 0.3 REACTOME ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT ESCRT Genes involved in Endosomal Sorting Complex Required For Transport (ESCRT)
0.0 2.9 REACTOME ANTIGEN PROCESSING UBIQUITINATION PROTEASOME DEGRADATION Genes involved in Antigen processing: Ubiquitination & Proteasome degradation